In the first subject by subject analysis we observed that CM from any adipose tissue fraction or depot elicited, in AG-881 manufacturer comparison to untreated cells (control) increased motility, independently of donnor’s clinicopathological characteristics (data not LY3039478 research buy shown). Figure 5 shows motile parameters of prostate cancer cells in response to adipose tissue CM. Comparing with control, LNCaP cells stimulated with CM from any fraction or depot always resulted in higher mean speed and final relative distance to origin (FRDO) (Figure 5A). In PC-3 cells, while mean speed was higher for any CM condition compared

with control, the FRDO was only increased after stimulation with CM from explants, both from PP and VIS depot (Figure 5B). Figure 5 Motility of PC3 and LNCaP cells upon stimulation of adipose tissue-derived CM from explants and SVF. Influence of adipose tissue fractions in cell motility parameters. Data represent mean ± SE of at least 20 representative cell trajectories per each tested condition, with conditioned selleck medium of primary adipose tissue cultures from four distinct subjects. Bars represent mean speed (MS) and plots the logarithmically transformed final relative distance to origin (FRDO). A. FRDO and MS of PC-3 cells (*** P < 0.0001 relative to control).

B. FRDO and MS of LNCaP cells (** P < 0.01 and *** P < 0.0001 relative to control). In the log-transformed FRDO we used one-way ANOVA with post-hoc Dunnett test (two-sided), whereas the mean speed was analyzed using Kruskal Wallis followed by Mann Whitney test. SVF, stromal-vascular fraction; PP, periprostatic; VIS, visceral. After adjustment of motility parameters to adipose tissue weight, in order to compare different culture types and depots, only the LNCaP cells mean speed was not statistically Glutamate dehydrogenase different between PP and VIS depot. Otherwise,

motile parameters were higher after stimulation with CM from PP depot (Figure 6). For both PC-3 (Figure 6A) and LNCaP (Figure 6B) cells stimulated with explant-derived CM from PP and VIS adipose tissue, the mean speed and FRDO were significantly higher in comparison to SVF (P < 0.0001). Figure 7 shows a representative example of cell tracking in both cancer cell lines, using CM from PP adipose tissue. Figure 6 Motility of PC-3 and LNCaP cells upon stimulation of adipose tissue-derived CM from explants and SVF. Data represent mean ± SE of at least 20 representative cell trajectories per each tested condition, from four distinct subjects. Bars represent mean speed (MS) per gram of adipose tissue and plots the logarithmically transformed final relative distance to origin per gram of adipose tissue (FRDO). A. FRDO and MS of PC-3 cells (* P < 0.05 and *** P < 0.0001 between treatment conditions). B. FRDO and MS of LNCaP cells (** P < 0.01 and *** P < 0.0001 between conditions).