202 0.653 17.3863 ± 6.67757 0.808* 0.370 No 7 30 18.4865 ± 6.97671 Alcohol consumption 0.608 0.436 0.008* 0.927 Yes 22 69 17.5388 ± 6.43099 No 22 90 17.6259 ± 6.99013 Location 2.213 0.331 3.550 0.031 Super glottic 24 69 18.2441 ± 7.14615 glottic 18 75 16.3786 ± 5.94319 subglottic 2 15 20.3667 ± 7.35727 pTNM Oligomycin A chemical structure 6.570 0.010 7.419* 0.007 I+II 11 74 16.0306 ± 6.19107 III+IV 33 85 18.5977 ± 6.91980 Tumor size (cm) 0.220 0.639 0.974* 0.325 ≥3 20 66
18.1306 ± 6.22807 >3 24 93 17.1872 ± 7.07416 T stage 1.278 0.734 3.396 0.019 T1 6 21 13.8593 ± 5.61853 T2 17 76 17.7731 ± 6.43417 T3 11 33 17.9143 ± 6.69789 T4 10 29 18.8667 ± 7.50099 Nodal status 9.097 0.003 0.019* 0.892 N-positive (N1, N2, N3) 19 33 17.4769 ± 6.50208 N-negative(N0) 25 126 17.6247 ± 6.82606 Distant metastasis 1.535 0.215 4.077* 0.045 Yes 2 17 20.4684 ± 6.86740 No 42 142 17.2186 ± 6.65992 Recurrence 0.005 0.994 0.679* 0.498 Yes 7 26 18.3152 ± 6.59413 No 37 133 17.4455 ± 6.76481 Histopathological grade 15.531 0.000 0.209 0.811 1 2 28 16.8967 ± 5.69443 2 30 119 17.7532
± 7.12289 3 12 12 17.4167 ± 5.42896 VM: vasculogenic ABT263 mimicry; EDV: endothelial dependent vessel; LSCC: laryngeal squamous cell carcinoma; TNM: tumor, node, metastasis. We performed immunohistochemical staining for CD31, a classic endothelial cell marker, to label endothelial dependent vessel, and analyzed whether it was associated with tumor clinicopathologic characteristic. The results showed MVD was correlated to location (p = 0.031), pTNM stage (p = 0.007), T stage (p = 0.019) and distant metastasis (p = 0.045). While, showed no
association between MVD and gender, age, tobacco use, alcohol consumption, tumor size, lymph node metastasis, recurrence or histopathological grade (all P > 0.05). Survival analysis Univariate analysis showed that survival of VM-positive patients was significantly poorer than that of VM-negative patients in OS (p = 0.014) (Fig. 2A). Furthermore, Idelalisib order pTNM stage (P = 0.009), T classification (P = 0.013), nodal status (P = 0.013), and histopathological grade (P = 0.038), tumor size (P = 0.028), radiotherapy (P < 0.0001) correlated with OS. However, there was no significant association between OS and gender, age at diagnosis, tobacco use, alcohol consumption, location, distant metastasis, recurrence and MVD (Fig. 2B) (all P > 0.05; Table 2). Multivariate analysis indicated that the Linsitinib molecular weight presence of VM (risk ratio (RR) = -2.117, P = 0.003), recurrence (RR = -1.821, P = 0.020) and pTNM stage (RR = 1.367, P = 0.009) were adverse predictors for OS (Table 3), while radiotherapy were indicators of a good prognosis of OS (RR = 2.872, P < 0.0001).