Additional material examined USA, Virginia, Blacksburg, on Celastrus scandens. 13 October 1936, C.L. Shear (BPI 615294). Notes: Diaporthe celastrina was originally described from Celastrus scandens in the USA (Kansas) and the epitype designated here is collected from the USA on the same host and also identified by L.E. Wehmeyer. The host Celastrus scandens (American Bittersweet, Celastraceae) is native to central and northeastern North America. Diaporthe helicis Niessl, Verh. Naturforsch. Ver., Brünn 16: 50 (1876). Fig. 7g–i [=Diaporthe nitschkei J. Kunze, Fungi Selecti Exs. 124. (1877), nom. nud.] Pycnidia on host and alfalfa twigs on WA 200–300 μm selleck inhibitor diam, globose, embedded in tissue, erumpent at maturity,
well developed, black stroma with a black, 50–150 μm long neck, often with an off white, conidial cirrus extruding from ostiole; walls parenchymatous, consisting of 3–4 layers of medium brown textura angularis. Conidiophores (6–) 8–15 (16.5) × 1–2 μm, hyaline, smooth, unbranched, ampulliform, cylindrical to clavate. Conidiogenous cells 0.5–1 μm diam, phialidic, cylindrical, terminal, tapering CP673451 in vitro slightly towards apex. Paraphyses absent. Alpha conidia (5.5–) 6–8 (9.5) × 2.5–3.5 μm (x̄±SD = 7 ± 0.5 × 3 ± 0.2, n = 30), abundant on alfalfa twigs, aseptate, hyaline, smooth, cylindrical to ellipsoidal, biguttulate or multiguttulate, base
subtruncate. Beta conidia not observed. Cultural characteristics: In dark at 25 °C for 1 wk, colonies on PDA fast growing, 5.6 ± 0.2 mm/day (n = 8), white, aerial mycelium turning to grey, reverse white, turning to grey in centre; stroma produced in 1 wk old culture Loperamide with abundant conidia. Host range: On vines and leaves of Hedera helix MDV3100 cost (Araliaceae) Geographic distribution: Europe (France, Germany) Type material: GERMANY, Saxony, Islebiam, on vines of Hedera helix, June 1875, J. Kunze (bound collection in BPI Joannes Kunze, Fungi Selecti Exsiccati 124, lectotype designated here; MBT178538, isolectotypes BPI 1108439; BPI 1108445); FRANCE, Veronnes, on vines of Hedera helix, 10 March 2011,
A. Gardiennet (BPI 892919, epitype designated here, ex-epitype culture AR5211 = CBS; MBT178539). Notes: When Niessl (1876) described Diaporthe helicis, he referred to the J. Kunze specimen that was distributed as J. Kunze, Fungi Sel. Exsiccati 124 labeled Diaporthe nitschkei. Although that exsiccati number was issued in 1875, the label does not include a description and thus that name was not published. The name D. helicis published 1 year later is typified by that same exsiccati number. Observations of the type specimens and additional material from Hedera confirmed that the fresh collection from France is D. helicis and belongs in the same species complex as does D. pulla described below. A comparison of representatives of D. helicis and D. pulla based on eight gene alignments and combined analysis revealed genetic differences suggesting that these two species are distinct. The third species on Hedera, D.