Both polyamines and NO have vital roles in cellular processes and cell signaling. protein inhibitor Nitric oxide and polyamines stimulate cell proliferation and have a positive effect on progression through the cell cycle. Polyamines exert their cellular effect through their ability to bind nucleic acids and proteins and have been demonstrated to promote an anti apoptotic state in vari ous cell lines. Moreover, NO can stimulate PI3K Akt 1 signaling pathways which promote cell Inhibitors,Modulators,Libraries survival. The role of L arginine and its metabolites in cell signaling has been studied extensively in ovine and porcine trophectoderm cells, with L arginine enhancing cell proliferation through mammalian target of rapamycin related signaling pathways. Wu et al. reported an unusual abundance of L arginine in porcine allantoic fluid, suggesting a role for this particular amino acid in fetoplacental nutrition.

Moreover, dietary L arginine supplementation Inhibitors,Modulators,Libraries has been demonstrated to enhance the reproductive performance of rats, pigs, and mice, and recently, we have shown that dietary L arginine supplementation increased the number of implantation sites in mice, which suggests an effect of L arginine at the level of the endometrium. The endometrium has the ability to catabolized L arginine in numerous species, including sheep, pigs, mice, rats, and humans, due to the presence of NOS and or arginase enzymes. Moreover, L arginine Inhibitors,Modulators,Libraries has been reported to exist in human uterine lumen flushes with the greatest concentration observed during the proliferative phase, suggesting a possible involvement Inhibitors,Modulators,Libraries in endometrial epithelial cell proliferation.

In the current study we used the human endometrial epithelial carcinoma cell line, RL95 2, Inhibitors,Modulators,Libraries as a model for endometrial epithelial cells. The RL95 2 cell line expresses markers found on normal human endometrial epithelial cells such as progesterone receptors, estrogen receptors and B, MUC1, and E cadherin. Furthermore, the RL95 2 cell line has been used extensively selleck products as an in vitro model for studying the human endometrial epithelium. To this end, the ob jective of this study was to examine the effect that L arginine may have on endometrial cell proliferation and apoptosis using the established human endometrial epi thelial cell line, RL95 2, as an in vitro model for epithe lial cells of the human endometrium. Methods Cell culture Human endometrial carcinoma cells were acquired from the American Type Cul ture Collection. Cells were cultured in a humidified incubator containing 5% CO2 using a complete growth media comprised of DMEM F12 media supplemented with 10% fetal bovine serum, 1% peni cillin streptomycin, and 0. 005 mg mL insulin in order to obtain frozen stocks.