By comparison with literature data, this component was ascertained as coniferin. By comparison together with the mass chromatography of FTZ as well as rat serum samples mGluR from management group, the MS spectra for rat serum samples from FTZ treated group exhibited 27 peaks in prevalent, which demonstrated that the 27 elements from FTZ were absorbed in to the rat blood immediately after oral administration. Moreover, there were a different nine peaks, which have been only detected in the dosed serum, indicating that those components were metabolites of constituents from FTZ. The MS spectra and retention conduct of 36 peaks for prototype parts and metabolites are summarized in Table 6. The constituents in rat serum after oral administration of FTZ were identied utilizing their retention time and mass spectra.
Like a end result, peaks 26 and 27 were original kind compounds present in Fructus Ligustri Lucidi, atm inhibitor peaks 18 came from Rhizoma Coptidis, peaks 21 and 23 resulted from Radix Notoginseng, peak 19 and 22 originated from Fructus Citri Sarcodactylis, peak 6 and 24 came from Cortex Eucommiae, peak 4 originated from Radix Salvia Miltiorrhiza. It displayed that most of alkaloids, ginsenosides and pentacyclic triterpenes could possibly be unambiguously detected in their unique varieties through the rat serum immediately after FTZ administration. To recognize the metabolites accurately, probable structures had been rst postulated in accordance with all the principles and characteristics of drug metabolic process in vivo. Within this review, the constituents of FTZ extract are identied. These information may well provide advice for investigating the metabolites of FTZ in rat serum.
M1 was identied as the glucuronide conjugate of alkaloids, jatrorrhizine3 O b D glucuronide, since it showed the m/z 514 in MS spectra, and exhibited m/z 338 in MS2 spectra, which was conrmed by Eumycetoma comparison with literature information. M2 and M3 have been suspected to be metabolite of ginsenoside Rh1/F1, the two of them showed precisely the same molecular ion at m/z 715 in MS spectra, and exhibited products ions m/z 655 and m/z 493 in MS2 spectra. By comparison with the literature information, this showed the exact same fragmentation pathway because the metabolite of ginsenoside Rh1/F1, so the 2 constituents were identied as the 25 hydroxyl ginsenoside Rh1/F1. Employing the same approach, M5 and M6 were identied as twenty / protopanaxatriol given that they showed the m/z 477 ion in positive ion mode and m/z 493 and m/z 553 ions Baricitinib 1187594-10-0 in detrimental ion mode. By comparison together with the literature information, we suggested that M5 and M6 may perhaps be sapogenin which formed by loss of all glycosidic units from protopanaxatriol saponins.