Good health perception, high level of albumin and white blood cell had positive effect on multiple domains of SF-36. Conclusion: Patients with PBC had impaired HRQOL. Age, female gender, present ascites and prolonged prothrombin time are important factors reducing HRQOL. Good health perception and high level of albumin may improve HRQOL. Key Word(s): 1. quality of life; 2. PBC; 3. SF-36; 4. influencing factor; Presenting MK-8669 purchase Author: MAYING JIE Corresponding Author: MAYING JIE Affiliations: Zhengzhou institute of liver and gastrointestinal disease Objective: To compare the differences
in immune effect of dendritic cell (DC) and cytokine-induced killer cell (CIK), which activated by HBsAg, in chronic hepatitis B (CHB) and healthy people. To investigate the potential effect of CHB patients. Methods: DCs and CIK cells were cultured and amplified from CHB and healthy people peripheral blood. DCs was stimulated with pure HBsAg in cell culture medium prior to maturation. ELISA was used to detect the level of IL-12 in the supermatants of co-cultured DCs and CIK cells. The cell-killing activity of DC-induced CIK cell against HepG2.2.15 cells was
measured. DC-CIK activated by HBsAg were reinfusion. Virus serological and Liver function were measured before and after 4,8,12 and 24 weeks of treatment. Results: the positive rate of HBsAg-activated DC and CIK cells surface selleck compound marker in healthy people were significantly higher than in CHB. The cell-killing activity of HBsAg-activated DC/CIK was significantly higher than non-activated in (-)-p-Bromotetramisole Oxalate CHB or healthy people (P < 0.05). The level of IL-12 in
supermatants of co-cultured HBsAg activated DC-CIK cells form healthy people was much higher than that form CHB (P < 0.001). HBsAg activated DC-CIK cell therapy for CHB, can reduce the viral replication at 24 weeks, viral response rate was 63.6%. Conclusion: surface markers and immune effects of DCs / CIK cells HBsAg-activated and non-activated form healthy people were significantly higher than CHB; whether form healthy people or CHB, DCs and CIK cells immune effector were enhanced by HBsAg-pulsed. Transfusion of autologous DCs/CIK cells activated by HBsAg inhibits viral replication in patients with CHB Key Word(s): 1. dc; 2. CIK; 3. immunotherapy; 4. CHB; Presenting Author: METIN BASARANOGLU Additional Authors: FATMA KALEM Corresponding Author: METIN BASARANOGLU Affiliations: Ankara YIH; Konya Numune Hospital Objective: Hepatitis B Virus (HBV) and Hepatitis C Virus (HCV) are very important infectious agents for public health. The aim of this retrospective study was to assess the seroprevalence of HBsAg, anti-HBs and anti-HCV test results of patients who admitted to first step health organizations in central and peripheral districts of Konya, the middle region of Turkey during the period 2005–2010.