However, our results also suggest that MtbPDF

is resistant to oxidative stress, as there was a >1000-fold increase in resistance compared with previously characterized Fe2+-containing E. coli PDF (Rajagopalan et al., Selumetinib molecular weight 1997b). Interestingly, G151D completely lost its activity upon incubating with 200 mM H2O2 (Fig. 3b). Thus, the increase in thermostability of G151D was accompanied by a decrease in oxidative stress resistance. The enzyme activity of MtbPDF was completely inhibited by 5 μM of the deformylase inhibitor actinonin, with an IC50 of 120 nM. Under similar assay conditions, G151D was completely inhibited with 10 μM of actinonin with an IC50 of 800 nM (Fig. 3c). This increase in IC50 of actinonin is a reflection of improved substrate affinity in the case of G151D. Other known metalloprotease inhibitors such as bestatin and amastatin did not produce any inhibitory effects in selleck products either case (data not shown). To analyse any possible secondary structure alterations induced by substitutions, the CD spectra of MtbPDF, G151D and G151A were compared. The far-UV-CD spectrum of MtbPDF had two typical negative minima at 208 and 222 nm with a crossover point at 198 nm

(Fig. 3d), indicating the presence of sheets and coils in addition to the predominant helical structure. The CD spectra of G151D showed a considerable amount of scatter to low mean residue ellipticity (approximately 30%; Fig. 3d). However, no shift in the negative minima at 222 or 208 nm was observed. These results indicated that the G151D mutation produced only restructuring in the less stable scaffolds such as turns and 310 helices, without affecting the α-helical fold. However, the CD spectrum of G151A was almost completely superimposable on that of MtbPDF. The overall structure

and stability of MtbPDF and G151D were examined by MD simulation. In the G151D model, D151 was not a part of the catalytic site and was located >50 nm from the metal ion (Fig. S1). The main chain root mean square deviation (RMSD) profile for the two structures (Fig. 4a) showed that G151D reached a flat profile after ∼100 ps whereas MtbPDF showed a variable profile during the entire simulation period. This demonstrated the higher stability of the G151D structure compared MtbPDF. The root mean square fluctuation (RMSF) plot of MtbPDF showed higher fluctuations in Loop DAPT research buy 1 (T22–D30) and the C-terminal loop (D191–H197) compared with G151D, whereas the latter showed greater fluctuations in Loop 6 (E91–T95) (Fig. 4b). The MtbPDF structure contains three α-helices, seven β-sheets and three 310 helices, forming three motifs and a structurally conserved active site (Pichota et al., 2008). Both MtbPDF and G151D had comparable secondary structures except that, in the latter, the first two 310 helices (12PVL14 and 53ANQI56) were transformed into turns. Additionally, the helix H1 started from A31 in G151D instead of D32 in MtbPDF.