In C. difficile it has been hypothesised that p-cresol is produced via the oxidation of tyrosine to p-HPA followed by the decarboxylation of p-HPA to p-cresol [15]. learn more However, the temporal production of p-cresol and its relative production among different C. difficile strains have not been investigated. Genome sequencing of the strain 630 (PCR-ribotype 012) suggests that the p-HPA decarboxylase is encoded by three genes (CD0153-CD0155)

designated hpdBCA [16]. However, the genes involved in the conversion of tyrosine to p-HPA are unknown. In this study we demonstrate the temporal and quantitative production of p-cresol by C. difficile in both minimal and rich media (Temsirolimus purchase supplemented with the intermediate p-HPA) using NMR spectroscopy and gas chromatography (zNose™). Gene inactivation mutations in the hpdA, hpdB and hpdC genes in strains 630Δerm and R20291 confirmed the absence selleck chemicals of p-cresol production in all mutants tested and conclusively show that tyrosine is converted to p-HPA by C. difficile under minimal media growth conditions. We show that R20291 is more tolerant to p-cresol and has a higher capacity

to convert tyrosine to p-HPA resulting in higher overall levels of p-cresol. Results Para-cresol tolerance and production The tolerance of strains 630 and R20291 to p-cresol was assessed in BHI broth as CFU counts per ml, expressed as a proportion of the untreated control for a four hour incubation period with 0.1% p-cresol (Figure 1). Strain R20291 (PCR-ribotype 027) showed a significant increase in survival to 0.1% p-cresol compared to strain 630 (PCR-ribotype 012) p < 0.01 using a Student's t-test (Figure 1). There was no significant difference in tolerance to p-cresol Paclitaxel in vitro between 630 and 630Δerm, an erythromycin sensitive spontaneous mutant (data not shown). The 630Δerm strain was essential to construct and select gene inactivation mutants for further investigations of p-cresol tolerance and production, therefore subsequent analysis was performed with the

630Δerm strain. Figure 1 Tolerance to p -cresol. Strains R20291 and 630 were tested for their in-vitro tolerance to 0.1% p-cresol. * indicates a significant difference p < 0.01 Student’s T-test. The production of p-cresol in-vitro was assessed in rich media using two complementary methods, NMR spectroscopy (Figure 2A) and zNose™ (Figure 2B). The production of p-cresol was not detected in the C. difficile strains 630Δerm or R20291 cultured to stationary phase in rich media (BHI broth, or BHI broth supplemented with cysteine) using either method, despite the availability of tyrosine (data not shown). However, when the strains were grown to stationary phase in rich media supplemented with 0.1% p-HPA, p-cresol was readily detected by NMR spectroscopy (Figure 2A) and zNose™ (Figure 2B) in both the 630Δerm and R20291 parent strains.