Now on the other hand, they are really used as constituents of composite materials for high tech applications. The robust, light-weight and very low value flax fibres are used as an alternative to standard glass fibres in reinforced plastic manufacture. Since they are biodegradable, their ultimate composite products is effortlessly recyclable. Really recently flax fibres application in bio medication is additionally beneath investigation. Their useful position as wound dressing continues to be reported In an effort to strengthen oil stability and to broaden seed cake and fibre application, mostly in medication, trans genic flax plant is created and analyzed. It had been expected that overexpression of 3 genes coding for important enzymes of flavonoid route will result in accumula tion of broad spectrum of antioxidant molecules in just about every a part of plant and so generating their standard solutions biotechnologically more precious.
The first stage while in the flavonoid route of the phenyl propanoid pathway will be the synthesis of naringenin chal cone by means of condensation of 3 malonyl CoA units with p coumaroyl CoA. The reaction is catalyzed through the action of chalcone synthase. The naringenin chal cone is actually a branch level for all flavonoid biosynthesis. Chalcones a total noob are incredibly labile compounds and may very well be accumulated as dihydrochalcones or swiftly converted for the colorless flavanone naringenin through the chalcone isomerase. Flavanones act being a precursor for your synthesis of flavones and 3 OH flavanones. Dihydroflavonols can then be converted into flavonols by FLS or into anthocya nins by DFR.
Because our gene construction that has been introduced into transgenic flax contained cDNAs for CHS, CHI and DFR enzyme, the flavonols inhibitor price and anthocyanins compound information was measured. The information obtained showed an increase in these compounds level in flaxseeds, seed cakes and fibres from plants of transgenic lines. It needs to be pointed out that measured flavonoid com lbs in seed may be than found in seedcake extract and hence suggesting that the residual tissues after oil extraction from seeds appeared for being ideal source of biomedical valuable compounds. It is actually also important to observe that generated transgenic flax are secure in respect to expression of exogenously launched genes because the area grown plants of third generation con tained all transgenes and they’re energetic which was con firmed by PCR examination of transgenic plant with complete mRNA as template. Also interesting is definitely the identification of elevated quan tity of phenolic acids and lignans in seedcake from transgenic seed. It is actually identified that these compounds like flavonoids are the constituents of phenylpropanoid path way however they are really biosynthesized on various meta bolic route.