Surviving fractions

were calculated as the CFU remaining

Surviving fractions

were calculated as the CFU remaining after UV exposure/total CFU present. Virulence determination of the rec mutants Eight-week old BALB/c female mice were purchased from Charles River Laboratories (Wilmington, MA). Mice were held in quarantine for 1 week before use in experiments. Food and water were deprived 6 h before administration of bacteria. Each mouse was orally inoculated with 20 μl of Salmonella suspended in buffered saline with gelatin (BSG) by pipet feeding. Food and water were returned 30 min after inoculation. All mice were observed for a month to record mortality. The 50% lethal dose (LD50) was determined via the Reed and Muench method [58]. Surviving mice were challenged orally with wild-type Salmonella χ3761 two months after the first inoculation. Acknowledgements This work was supported by grants from the National Institutes of Health (AI065779) and the Bill learn more & Melinda Gates Foundation (no. 37863). References 1. Levine MM, Ferreccio C, Abrego P, Martin OS, Ortiz E, Cryz S: Duration of MK-8931 manufacturer efficacy of Ty21a, attenuated Salmonella Typhi live oral vaccine. Vaccine 1999,17(Suppl 2):S22–27.PubMedCrossRef 2. Curtiss R III: Bacterial infectious disease control by vaccine development. J Clin Invest 2002,110(8):1061–1066.PubMed 3. Tacket CO, selleck chemicals llc Levine MM: CVD 908, CVD 908-htrA, and CVD 909 live oral typhoid vaccines: a logical

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