The analysis of REP profiles suggest the existence of 2 clones. Selleckchem AUY-922 Clone A included 2 strains from sampling time F4 (F4-42 and F4-44), isolated from a sink and a tap, and from sampling time F3 (F3-6) also from a tap but from a different ward. Clone B included two strains (F4-6b and F7-6a) from different sampling times (F4 and F7) isolated from the same tap (Additional file 1: Figure S1). Table 2 Diversity of bacteria isolated and identified by 16S rRNA gene sequencing

  Samples showing fluorescence by month and year Organisms isolated (number of strains) Month/Year F 10 A 10 J 10 O 10 D 10 F 11 M 11 J 11 S 11   Sink 3 6 4 4 7 16 8 9 10 Acinetobacter pittii Bacillus aryabhattai Citrobacter braakii Citrobacter freundii Enterococcus faecalis Pseudomonas aeruginosa (10) Pseudomonas beteli* Pseudomonas hibiscicola Tideglusib Pseudomonas monteilii Pseudomonas mosselii Pseudomonas plecoglossicida Pseudomonas putida Pseudomonas taiwanensis Serratia nematodiphila Sphingobium yanoikuyae (2) Stenotrophomonas maltophilia (3) Stenotrophomonas rhizophila Tap – 3 3 5 9 5 8 7 7 Citrobacter

braakii Enterococcus faecalis (2) Erwinia aphidicola Neisseria subflava Pseudomonas aeruginosa (16) Pseudomonas hibiscicola Pseudomonas monteilii Serratia nematodiphila (2) Stenotrophomonas maltophilia (6) Shower (Handrail) 1 1 2 1 1 1 – 2 – Pseudomonas aeruginosa (2) Pseudomonas plecoglossicida Pseudomonas monteilii Hand Gel (soap) – - 1 – 3 – - – - Pseudomonas aeruginosa Pseudomonas beteli* Shewanella oneidensis Citrobacter freundii Workbench/ S. countertop 1 1 – 4 – - 1 – - Pseudomonas aeruginosa Pseudomonas beteli* Tray – - 2 – 2 – - – 2 Pseudomonas aeruginosa Bedside Table 1 – 2 – 1 – - – - Pseudomonas aeruginosa (2) Pseudomonas beteli* Pseudomonas monteilii Bedside equipment – - – - – - – 1 – Pseudomonas aeruginosa Table (work/meal) – 1 – - – - 1 – 1 Pseudomonas alcaligenes PIK3C2G Pseudomonas putida (*- bacterial species

isolated in different equipment). The isolation of strains from the species P. aeruginosa was expected since the isolation conditions favoured its ARRY-438162 datasheet recovery. However, Stenotrophomonas maltophilia, Enterococcus feacalis, Sphingobium yanoikuyae and Serratia nematodiphila were also repeatedly isolated on the same equipment, on different times. Seven different species of Pseudomonas were isolated on the sinks surfaces. Some of these species were also isolated on other surfaces as P. beteli on hand gel/soap, workbench and bedside table. P. montelli was also isolated on the sink surfaces, taps, showers and bedside tables. Some of the organisms isolated were already reported as pathogenic. This is the case of Citrobacter braakii, C. freundii, E. faecalis, P. mosselii, P. putida, S. maltophilia, Neisseria subflava, P. alcaligenes or isolated from hospital environment as P. monteilii.