The complete sequences were identical to that published for S. aureus COL (ST250), which is a close relative of the Iberian strain, and S. aureus RF122. The promoter sequence of the cap5 gene cluster and the inverted repeats that constitute the operator [58, 59] were identical to that of the first seven published genomes. Unexpectedly, the control strain SA1450/94 showed an insertion of IS256 into the first gene of the capsule gene cluster cap5A1. The IS element was located 50 bp downstream of the ATG start codon and oriented in an antisense direction. Cap5A1 encodes a membrane protein that is part of the protein kinase Cap5A1/Cap5B2, which

Selleck EPZ 6438 is needed for phosphorylation of Cap5O [60]. In spite of this, in in vitro experiments Cap5A1 is not essential for activation of Cap5O since a paralogue of Cap5A1, Cap5A2 is encoded by SA2457 and able to activate the kinase subunit Cap5B2 [60]; this is VX-770 solubility dmso also demonstrated by the fact that SA1450/94 was able to produce capsule, albeit at low levels,

in overnight cultures (data not shown). The effect of capsule on vancomycin resistance in VISA Initial attempts to knock out capsule production in the VISA strains resulted in mutants that could not be complemented because they harboured background mutations in regulatory genes that are necessary for capsule production and influence glycopeptide susceptibility (rsbU, agr), e.g., inactivation of rsbU led to an increase in vancomycin susceptibility in our isolates even if capsule biosynthesis had been reconstituted. Therefore, we chose an antisense approach. An N-terminal 166 bp fragment of cap5D was ligated to pEPSA5 in antisense direction and transformed into S. aureus 137/93G. We chose another region than that described in [30] since antisense RNA expression from this fragment had exerted

growth-inhibitory effects. Capsule formation was analyzed by immunofluorescence in the absence and presence of 50 mM PD184352 (CI-1040) xylose in different media (LB, BHI and CYPG [61]) after 6 h of incubation. Figure 4 shows that after only 6 h of incubation, capsule formation in the wildtype SA137/93G is relatively strong even in LB (Figure 4c), and that the capsule formation is somewhat decreased in the presence of the plasmid even in the absence of xylose (Figure 4b). Addition of 50 mM xylose (but not 12.5 mM) led to a full repression of capsule biosynthesis (Figure 4c) in all tested media with the exception of a few cells that had obviously been able to see more eliminate the plasmid. Figure 4 Suppression of capsule formation by expression of cap5D -antisense RNA. CP5 was labelled by immunofluorescence (CY3, green), the cells were stained using DAPI (blue). Cells were grown for 6 h in LB at 37°C. a) S. aureus SA137/93G (control); b) S.