The mean recovery of PZA from plasma

spiked samples of PZA, in terms of LQC, MQC and HQC levels were respectively, 27.99%, 26.52% and 27.13%. The overall recovery of PZA was 27.21% with a coefficient of variation of 2.71% (n = 6). Internal standard recovery at 200 μg/ml of MTZ was 83.34% with a coefficient of variation of 4.38%. A HPLC method was developed and validated for the determination of PZA in human plasma. The extraction process was a single-step liquid–liquid extraction (LLE) procedure employing the use of 70:30% v/v of t-butyl methyl ether and dichloromethane. LLE method is usually devoid of polar interferences thus rendering the sample clean for final HA1077 analysis. The noise is usually absent or at minimum as compared to precipitation or SPE techniques. This assay requires only a small volume of plasma (500 μl). There is no carryover effect. Due to the LLE method of extraction, baseline noise is minimal. Matrix effects are not observed. In conclusion, method validation following FDA guideline

indicated that the developed method has high sensitivity with an LLOQ of 1.02 μg/ml, acceptable recovery, reliability, specificity selleck chemicals llc and excellent efficiency with a total running time of 8.0 min per sample, which is important for large batches of samples. Thus this method can be suitable for pharmacokinetic, bioavailability or bioequivalence studies of PZA in human subjects. This method has been successfully applied to analyze PZA concentrations in human plasma. All authors have none to declare. This authors wish to thank the Department of Science and Technology, New Delhi, India for granting research fellowship under DST-PURSE Programme, to carry out this work. The authors also wish to express

their gratitude to M/s Lupin Pharma Pvt Ltd for supplying the gift sample of pyrazinamide. “
“Invasive fungal infections, particularly in immunosuppressed patients, have continued to increase in incidence during the past 20 years and are now significant causes of morbidity and mortality.1 Long before mankind discovered the existence of microbes, the idea that various synthetic compound had during healing potential, that they contained what we would currently characterize as antimicrobial principles, was well accepted. Since antiquity, man has employed the synthetic to treat common infectious diseases and some of these traditional medicines are still included as part of the habitual treatment of various maladies.2 Autopsy data indicate that more than half of the patients who die with malignancies are infected with Candida spp., approximately one-third with Aspergillus spp., and increasing numbers with Cryptococcus spp. or other fungi such as Fusarium spp.