The TCR of CD8 T cells recognize only class I MHC-antigen

complexes, while those of CD4 T cells recognize only class II MHC-antigen complexes. T cell activation is optimal when MHC-antigen complexes are presented by professional APC, comprised of activated B cells and macrophages and mature dendritic cells (DCs), because these APC coexpress costimulatory molecules CD80 and CD86. Binding of CD80 and CD86 to the CD28 receptor on both naïve CD4 and CD8 T cells delivers a costimulatory signal required for functional differentiation into CD4 T cell subsets and CD8 CTL (Fig. 1). Treg cells are indispensable for maintenance of self-tolerance and regulation of the extent and duration of normal immune responses. T cell precursors traffic to the thymus, where those with TCR with high affinity CHIR-99021 solubility dmso for autoantigens are deleted.8 During thymic development of CD4 T cells, a variable proportion express repressor forkhead winged helix ABT-737 research buy transcription factor box (FoxP3) and later differentiate into natural CD4 Treg cells with the phenotype CD4+CD25+highCD45RO+highCD62L+highCD127lowFoxP3+high, where “high” refers to

the degree of expression, CD25 is the receptor for the α-chain of the T cell mitogenic cytokine IL-2, CD62L is a lymph node homing receptor, and CD45RO

is an activation marker in humans.4, 6 While this phenotype is similar for natural and iTreg, iTreg are functionally less stable. Of potential importance, FoxP3 expression is subject to epigenetic control, indicating that a functionally altered gene can be inherited by progeny cells.9 Furthermore, it is now clear that T effector cells also express low levels of FoxP3; thus, FoxP3 expression alone cannot 上海皓元医药股份有限公司 be used to define Treg cells.6 In addition to classical CD4+CD25+FoxP3+ Treg cells, suppression of autoimmunity and regulation of normal immune responses are also mediated by CD4 interleukin (IL)-10-secreting T regulatory 1 cells (Tr1), CD4 transforming growth factor β (TGFβ)-secreting Th3 cells, CD8 T suppressor cells, and some natural killer T (NKT) cells (Fig. 1).4 The central role of natural Treg in maintenance of self-tolerance was confirmed by evidence that their deficiency results in fatal autoimmune diseases and chronic inflammation with lymphoproliferation (4, 6). In humans, mutation of FoxP3 causes Treg deficiency and the immune dysregulation, polyendocrinopathy, enteropathy, X-linked (IPEX) syndrome. In mice, a naturally occurring FoxP3 mutation also causes systemic autoimmunity, which can be reproduced experimentally by deletion of Treg cells.