The UV spectra of every peak were related with maxima at 300 nm and about 250. The HPLC conditions were chosen based on the chromatography of tanshinones. The retention times were custom peptide price much like printed retention times for tanshinones. The UV spectra were just like published spectra for miltionones, cryptotanshinone and related compounds. The extinction coefficients of tanshinone IIA are lambamaxMeoH nm : 220, 250 and 269,. Based on the similar UV spectra and similar chromophores of the three compounds, the extinction coefficients are probably similar for each. The HPLC peaks for the three substances integrated as follows: miltionone II 4. 2 min 25. 2%, cryptotanshinone, 6. 9 min 69% and tanshinone IIA, 10. 2 min 5. 8%. The levels of each element in the 50 ml extract were: miltionone II, 0. 7mol, cryptotanshinone, 2 mol, and tanshinone IIA, 0. 2 mol. An HPLC?MS chromatogram is shown in Figure 3. Chia was grown at the Rancho Santa Ana Botanic Garden, Claremont, FLORIDA. The entire plants were gathered, when the plants were about 1 m tall. At this time, they certainly were in flower with large seed clusters. The flowers were put into plastic bags and kept AG-1478 clinical trial in a freezer. Chia could be difficult to develop to maturity. The vegetables sprout sometimes generously, but Infectious causes of cancer die quickly or even in the environment. The seeds were found to grow most readily useful completely shade, with lots of water, good drainage and the effective use of calcium if the plants are about 2 cm tall. Transplanting the seedlings into lime containing earth resulted in the loss of most plants. Chia was found to contain 17. 5mol of tanshinone IIA per kilogram of root material. This is three fold less than is found in dan shen. However, chia includes not quite fivefold more cryptotanshinone than is situated in john shen. Cryptotanshinone is just a precursor for tanshinone IIA and is converted into tanshinone purchase Dizocilpine IIA in the liver. This means that chia contains 192. 5 mol/kg of active tanshinones. John shen includes 91 mol/kg of active tanshinones. The presence of tanshinone IIA and similar substances in chia could describe the old use of this plant, to wake the dead, or the almost dead such as for instance with heart and stroke attack patients. Tanshinones have a range of pharmacological activities including inhibition of clotting, vasodilatation and inhibition of NO synthase. Many of these activities are potentially beneficial in stroke. Swing is generally due to blood clots that dislodge in one place and travel in the blood system until they settle in small cerebral arteries. This causes brain ischemia and frequently influences more clotting in the area. Inhibition and vasodilatation of clotting may help dislodge and dissolve the clot.