To ensure that participants carefully processed the critical target words, a paper–pencil postscanning recognition-test was administrated

outside the scanner after the completion of the main experiment. The recognition-test was composed of 240 words. Among these words, 30 words were critical target words of the experiment (“old” target words, 1/8) whereas, the other 210 words were not (“new” target words). For each word, participants were told to indicate whether Inhibitors,research,lifescience,medical this word was presented during the experiment (“old” word) or not (“new” word). The first session was preceded by a short practice session of 12 items before scanning started. Practice was repeated once if participants did not understand the task. Each of the five sessions lasted for ~10 min, with 1–2 min rest between each session. Behavioral data analysis Experiment 1 A counter module was started at the onset of the visual target presentation to register RT using presentation (Neurobehavioral Systems). Inhibitors,research,lifescience,medical We recorded both reaction times (RTs in msec) and accuracy (in %). Time-out was set at 200 msec and at 1800 msec; if the participants responded before 200 msec or after 1800 msec, the response was coded as missing. A correction procedure (mean ± 2SD)

was applied on the RTs for correct responses in order to discard extreme values. RTs were then averaged in the two buy U0126 experimental conditions across participants Inhibitors,research,lifescience,medical and across items. Priming effects were calculated by subtracting the averaged RT in the related condition from the averaged RT in the unrelated condition by participants and by items. Experiment 2 The postscanning recognition-test resulted in accuracy rates that are indicated by the percentage of hits (percentage of “old” words that were correctly recognized as “old”) and of correct rejections Inhibitors,research,lifescience,medical (percentage of “new” words that were correctly identified as “new”). We computed the mean percentage of hits and the mean percentage of correct rejections

of the postscanning Inhibitors,research,lifescience,medical recognition-test per participant to gain accuracy rates. fMRI acquisition and analysis All imaging data were collected with a 3.0-Tesla Magnetom TrioTim syngo MR B13 whole body system (Siemens, Erlangen, Germany). Image acquisition consisted of a fast T1-weighted sequence (localizer) and T2*-weighted sequences for functional images. Functional images were acquired in 38 axial slices using a Liothyronine Sodium BOLD-sensitive gradient-echo echoplanar imaging (EPI) sequence with an echo time (TE) of 30 msec, a flip angle of 90 degrees, a TR of 2.37 sec, and an acquisition bandwidth of 100 kHz. The matrix acquired was 64 × 64 with a field of view (FOV) of 192 mm2, resulting in an in-plane resolution of 3 mm × 3 mm. Slice thickness was 3 mm without interslice gap. Each trial had a length of 2.7 sec followed by an ITI in milliseconds varying from 2000 msec to 2000 msec + 1 TR. The functional measurements were carried out in five sessions of about 10 min length.