1.3.48 K. pneumoniae strain MGH 78578 (ABR77929) (78%) KP03806 2,154 35.61 wzc Uncharacterized tyrosine-protein kinase 2.7.10.- K. pneumoniae strain MGH 78578 (ABR77928) (79%) KP31533 1,446 35.2 wbaP

Undecaprenolphosphate Gal-1-P transferase 2.-.-.- K. pneumoniae strain MGH 78578 (ABR77927) (79%) KP03804 906 37.51 orf8 Uncharacterized selleck kinase inhibitor glycosyltransferase family 2 2.4.1- K. pneumoniae strain A1517 (BAF75773) (67%) KP03803 894 30.99 orf9 Uncharacterized glycosyltransferase family 2 2.4.1- Dickeya dadantii (ADM97617) (63%) KP03802 759 29.79 orf10 Uncharacterized glycosyltransferase 2.4.1.- D. dadantii (ADM97619) (57%) KP31534 1,404 51.46 gnd 6-phosphogluconate dehydrogenase, decarboxylating 1.1.1.44 K. pneumoniae strain VGH484 serotype K9 (BAI43786) (99%) KP31530 1,062 59.25 rmlB dTDP-D-glucose 4,6-dehydratase 4.2.1.46 K. pneumoniae strain VGH484 serotype K9 (BAI43787) (98%) KP03797 867 58.74 rmlA Glucose-1-phosphate thymidylyltransferase 2.7.7.24 Escherichia coli HS (EFK17576) (98%) KP03796 888 61.5 rmlD dTDP-4-dehydrorhamnose reductase 1.1.1.133 K. pneumoniae strain MGH 78578

(ABR77913) (98%) KP03795 552 54.41 rmlC dTDP-4-dehydrorhamnose 3,5-epimerase 5.1.3.13 K. pneumoniae strain VGH484 serotype K9 (BAI43790) (99%) KP03794 1,164 50.82 ugd UDP-glucose 6-dehydrogenase 1.1.1.22 K. pneumoniae strain NK8 (BAI43716) (100%) and strain VGH404 serotype K5 (BAI43755) (100%) KP03793 999 41.92 uge-1 Uridine diphosphate galacturonate 4-epimerase 5.1.3.6 K. pneumoniae subsp. rhinoscleromatis ATCC 13884 (EEW43608) (97%) KP31531 1,233 31.57 wzx K-antigen flippase Wzx   E. coli TA27 (ZP_07523140) (64%) KP03791 990 31.32 Gemcitabine mw orf19 Uncharacterized glycosyltransferase family 2 2.4.1.- Cronobacter sakazakii (ABX51890)

(33%) KP03789 1,044 29.61 wzy K antigen polymerase Wzy   Thermoanaerobacter wiegelii (ACF14522) (35%) The cps Kp13 has a genomic organization similar to other K. pneumoniae cps clusters, and it can be divided into three regions as shown in Figure 1. The 5’ end or region 1 (from galF to wbaP) contains conserved genes responsible for polymer assembly and translocation [12]. The central region or region 2 contains genes encoding serotype-specific GTs and gnd. The 3’ end or region 3 is more variable among different capsular types, with some containing the Dapagliflozin manCB operon that encodes GDP-D-mannose, like serotypes K1 and K5 [15]. Similarly to serotypes K9 and K52, the 3’ end of the cps Kp13 gene cluster contains the rmlBADC operon for the synthesis of dTDP-L-rhamnose instead of the manCB operon [15]. The genes wzx and wzy are also found in the 3’ region of the Kp13 cps cluster. This region is succeeded by defective IS elements and a prophage fragment (Figure 1). The discussed conservation of region 1 and variability of region 2 can be readily observable on a comparison of the cps loci of different K-types deposited in NCBI (Figure 2). Figure 2 Comparison of sequenced  K. pneumoniae cps  loci.