2% and an HSP coverage of 99.9%. The most frequently occurring keywords within the labels of all environmental selleck chemicals Cabozantinib samples which yielded hits were ‘marine’ (3.0%), ‘lake’ (2.9%), ‘depth’ (2.7%), ‘water’ (2.6%) and ‘zone’ (2.5%) (249 hits in total) and corresponded with the habitat from which strain UST20020801T was isolated. Figure 1 shows the phylogenetic neighborhood of O. hongkongensis in a 16S rRNA based tree. The sequences of the two identical 16S rRNA gene copies in the genome do not differ from the previously published 16S rRNA sequence (“type”:”entrez-nucleotide”,”attrs”:”text”:”AB125062″,”term_id”:”38093231″,”term_text”:”AB125062″AB125062). Figure 1 Phylogenetic tree highlighting the position of O. hongkongensis relative to the type strains of the other species within the family Cryomorphaceae.

The tree was inferred from 1,409 aligned characters [21,22] of the 16S rRNA gene sequence under the maximum … Table 1 Classification and general features of O. hongkongensis UST20020801T according to the MIGS recommendations [29] and NamesforLife [30]. O. hongkongensis UST20020801T is a Gram-negative, halophilic, non-flagellated, motile, and rod-shaped bacterium (Figure 2) [1]. Colonies are orange, convex, smooth, glistening and translucent with an entire margin [1]. Cells are 0.3-0.5 ��m in width and 0.5-4.0 ��m in length [1]. The strain does not sporulate [1]. Cells are strictly aerobic heterotrophs requiring Na+, Mg2+, sea salts and yeast extract or peptone for growth [1]. Growth occurs between 4��C and 37��C with an optimum at 25��C-33��C [1]. The pH range for growth is 5.

2-9.0 with an optimum at pH 6.0-8.0 [1]. The salinity range for growth is 1.0-7.5% NaCl as well as 15-100% sea-water [1]. Yeast extract, peptone or starch is required for growth [1]. Ampicillin (10 ��g), chloramphenicol (30 ��g), erythromycin (10 ��g), penicillin G (2U), rifampicin (10 ��g), streptomycin (10 ��g), tetracycline (30 ��g) and polymyxin B (300 U) inhibited Cilengitide growth whereas cells were resistant to kanamycin (10 ��g), gentamycin sulphate (10 ��g) and spectinomycin (10 ��g) [1]. Cells contain oxidase, catalase and alkaline phosphatase [1]. Figure 2 Scanning electron micrograph of O. hongkongensis UST20020801T Chemotaxonomy The fatty-acid profile of strain UST20020801T differs significantly from those of other members of the Cryomorphaceae [1]. The principal cellular fatty acids of strain UST20020801T were the following saturated branched-chain fatty acids: iso-C15:0 G (28.0%), iso-C15:0 (18.7%), iso-C17:0 3-OH (18.1%), iso-C17:1 ��9c (7.3%), iso-C15:0 3-OH (4.9%), and a summed feature containing iso-C15:0 2-OH and/or C16:1��7c (10.0%) [1].