enterica serovar Typhi P-stx-12, S. enterica serovar Typhi CT18, and S. Ganetespib buy enterica serovar Typhi Ty2 CRISPR Region By using the CRISPR Finder tool, one CRISPR repeat region with a length of 394 bp was identified in the S. enterica serovar Typhi P-stx-12 genome. The CRISPR region starts at the position 2,900,675 and ends at the position 2,901,069 with 6 spacers in between. The confirmed CRISPR has the following direct repeat consensus sequence: CGGTTTATCCCCGCTGGCGCGGGGAACAC. Strains CT18 and Ty2 also have a single CRISPR repeat region with the lengths of 385 bp and 394 bp, respectively. The location for the CRISPR region of all three strains falls within the region of 2.9 Mbp on the chromosome. All the strains have 6 spacers and share the common direct repeat consensus sequence.

It is worth noting that the CRISPR region, including the length and the spacer sequence, of S. enterica serovar Typhi P-stx-12 is exactly identical to S. enterica serovar Typhi Ty2. It suggests a strong evidence of their evolutionary relevance and shows that the CRISPR region in S. enterica serovar Typhi is conserved. As CRISPRs function as a prokaryotic immune system and confer resistance towards plasmids and phages (thus interfering with the spread of antibiotic resistance and virulence factors), it is reasonable to find only one CRISPR with very few spacers in this pathogen as compared to other bacterial strains that are not pathogenic [49]. Acknowledgement This work was supported by APEX funding (Malaysia Ministry of Higher Education) to the Centre for Chemical Biology, Universiti Sains Malaysia; and the contributions of the Council of Scientific and Industrial Research (CSIR), New Delhi, India.

Notes Abbreviations: NCBI- National Center for Biotechnology Information RDP- Ribosomal Database Project
Strain YIM 70093T (= DSM 44683T) is the type strain of the species Corynebacterium halotolerans [1] and was originally isolated from saline soil in Xinjiang Province in western China. The genus Corynebacterium is comprised of Gram-positive bacteria with a high G+C content. It currently contains over 80 members [2] isolated from diverse backgrounds like human clinical samples [3] and animals [4], but also from soil [5] and ripening cheese [6]. Within this diverse genus, C. halotolerans has been proposed to form a subclade together with C. freneyi and C. xerosis [1].

Data concerning salt tolerance is not available for most corynebacteria, but C. halotolerans YIM 70093T displays the highest resistance to salt (up to 25%) GSK-3 described for Corynebacterium so far. Here we present a summary classification and a set of features for C. halotolerans YIM 70093T, together with the description of the genomic sequencing and annotation. Classification and features A representative genomic 16S rRNA sequence of C. halotolerans YIM 70093T was compared to the Ribosomal Database Project database [7], confirming the initial taxonomic classification.