Data from the current investigation show that in the P. lanceolata Selleckchem NVP-BKM120 system, ‘background’ microbial richness and community structure in addition to AMF status were important positive determinants of aggregate stability. The minimal difference observed in aggregate stability between the NM planted soil and the bare soil is interesting. Aggregate stability was greater in mycorrhizal soils overall, although this was not the case in every month of the experiment, suggesting that aggregate stability is dynamic. Furthermore across all months, bacterial TRF richness was positively correlated with aggregate stability in the bare soil and the NM planted

treatments. In contrast, bacterial TRF richness was negatively correlated with aggregate stability in the mycorrhizal soils, where it is possible that bacterial richness was reduced by extraradical AM fungal hyphae or glomalin production. Neither of these parameters

was measured here, although they Selleck Erastin are both known to increase hydrophobicity in aggregates. However, the system was more complex than the correlations using all the data might suggest, since dilution and month affected bacterial richness in the AM treatments. In the present study, both aggregate stability and repellency were reduced in month 7; specifically the degree of reduction in repellency was less in the mycorrhizal soils than in the non-mycorrhizal soils. In the mycorrhizal soils, aggregate water repellency was also negatively correlated with bacterial (and fungal) TRF richness but positively correlated with root size and microbial biomass-C. It is likely that mycorrhizal hyphae contributed to the microbial biomass-C measured here which might explain why microbial biomass-C was not a factor in the model explaining repellency in the NM soils. In the mycorrhizal soils

the relationship between microbial biomass-C and aggregate stability was negative, whilst it was positive for repellency. The GLM regressions used data for all 7 months but the system was dynamic across the months. For example, aggregate stability was greater in the mycorrhizal soils in month 3, yet repellency increased in months 5 and 7. The positive relationship observed between per cent root length colonised and microbial biomass-C is likely to be the result of increasing hyphal length in the soil, Amobarbital or possibly an enhancement of other microbial species too, since internal AMF root colonisation may not reflect the extraradical hyphal biomass. Aggregate turnover rates range from 4 to 88 days (De Gryze et al., 2005 and De Gryze et al., 2006); an increase in aggregate stability observed here over a 60 day period (from the first to third month harvest) and an increase in aggregate water repellency over a 120 day period (from the first to fifth month harvest) is comparable to that observed by others. Mycorrhizal colonisation resulted in reduced plant growth and therefore less root material in the soil. In the tomato study conducted by Hallett et al.