The excitonic peak energies corresponding to the ground electronic subband and the ground heavy-hole band (E-1-HH1) at several temperatures, as determined from the FDM calculations taking into account strain effects, were in qualitatively reasonable agreement with those corresponding to the (E-1-HH1) excitonic transition, as determined from the temperature-dependent photoluminescence spectra.”
“The objective of this study was to investigate serum lipids, metabolic Go 6983 mouse parameters and activity of the anti-oxidative enzyme paraoxonase-1 (PON1). The study was conducted on non-pregnant heifers with

optimal health status and on healthy dairy cows in the period of intensive lactation, assuming that the energy and metabolic demands during lactation reduce anti-oxidative protection. Total cholesterol and HDL-cholesterol concentrations were significantly higher (P < 0.05) in lactating cows than in heifers. Bilirubin concentration and gamma-glutamyltransferase

(GGT) activity were also significantly higher in lactating cows (P < 0.05), indicating increased hepatic efforts of cows to meet energy requirements for lactation. Significantly lower PON1 activity and PON1/HDL ratio in lactating cows compared to heifers (P < 0.05) showed that metabolic efforts during pregnancy, parturition and lactation influence PON1 activity due to oxidative stress. Concurrent increase in total and S3I-201 HDL-cholesterol during lactation indicated that the HDL particle is a major carrier of cholesterol in cows. (C) 2010 Elsevier Ltd. All rights reserved.”
“Oral dasatinib (Sprycel(A (R))) is effective in the treatment of patients with newly diagnosed chronic-phase chronic myeloid leukemia (CML) or imatinib-resistant or imatinib-intolerant chronic-phase, accelerated-phase, or blast-phase CML. In particular, dasatinib is associated with better and faster responses than imatinib in patients with newly diagnosed chronic-phase CML. The response to dasatinib appears to be maintained in the longer-term. Dasatinib has an acceptable tolerability profile; it is associated SN-38 with myelosuppression,

with fluid retention being the most common nonhematologic adverse event. Thus, dasatinib is a highly effective, once-daily therapy for the first-line treatment of patients with chronic-phase CML and for the second-line treatment of imatinib-resistant or imatinib-intolerant CML.”
“In the past investigations, the exchange-biased bilayers show much larger ferromagnetic resonance (FMR) linewidth than that of single ferromagnetic layer films. However, the mechanism of the large linewidth remains controversial. In this paper, the FMR linewidths of NiFe/FeMn bilayers prepared by dc magnetron sputtering system are systematically studied. Besides the intrinsic damping and magnetic inhomogeneity, the extrinsic relaxation based on two-magnon scattering process should be also considered to explain the strong in-plane angular dependence of the linewidths.

This study examined the impact of several variables on combined all-cause mortality and hospitalization for cardiac causes, in consecutive ambulatory patients with HF included in the ALPHA registry.

Methods and Results: This analysis included 446 patients with HF and nonischemic heart disease, in New York Heart Association functional class II or III, and a left ventricular (LV) ejection fraction below 40%. In 126 patients (73%) the disease was idiopathic dilated cardiomyopathy, in 72 (16%) hypertensive, in nine (2%) valvular, and in 39 (9%) of other etiologies. The median age was

61 years (range 51-69 years) and 349 (78%) patients were men. Over a median follow-up of 31 Immunology & Inflammation inhibitor months (range 23-40), 82 patients (18%) died or were hospitalized for cardiac causes. In a proportional hazard (Cox) regression model, maximal oxygen consumption (HR 0.9, P = 0.001), LV end-diastolic diameter (HR 1.07, P < 0.001), resting systolic blood pressure (HR 0.97, P < 0.005), and hemoglobin (HR 0.86, P < 0.05) were independent predictors of

the combined study endpoint.

Conclusions: In an unselected population of patients with HF and nonischemic heart disease, a reduced exercise capacity, large LV end-diastolic Z-VAD-FMK order diameter, low systolic blood pressure, and hemoglobin were correlated with long-term all-cause mortality or hospitalization for cardiac causes. These observations may help stratifying and tailoring the treatment of patients with HF and nonischemic heart disease. (PACE 2009; 32:S214-S218)”
“BACKGROUND: Interferon-gamma release assays (IGRAs) are reported to be more specific for the diagnosis of latent tuberculous infection (LTBI) than the tuberculin Bcr-Abl inhibitor skin test (TST). The two-step procedure, TST followed by an IGRA, is reported to be cost-effective in high-income countries, but it requires more financial resources.

OBJECTIVE: To assess the added value of IGRA compared to TST alone in the Netherlands.

METHODS: Test results and background data on persons tested with an IGRA were recorded by the Public Municipal Health Services in a web-based database.

The number of persons diagnosed with LTBI using different screening algorithms was calculated.

RESULTS: In those tested with an IGRA, at least 60% of persons who would have been diagnosed with LTBI based on TST alone had a negative IGRA. Among those with a TST reaction below the cut-off for the diagnosis of LTBI, 13% had a positive IGRA. For 41% of persons tested with an IGRA after TST, the IGRA influenced whether or not an LTBI diagnosis would be made.

CONCLUSION: With the IGRA as reference standard, a high proportion of persons in low-prevalence settings are treated unnecessarily for LTBI if tested with TST alone, while a small proportion eligible for preventive treatment are missed. Incremental costs of the two-step strategy seem to be balanced by the improved targeting of preventive treatment.

Kinetics ITF2357 cell line of drug release followed the Higuchi square root equation, indicating that release is diffusion-controlled. (C) 2010 Wiley Periodicals, Inc. J Appl Polym Sci 117: 1361-1370, 2010″
“Background: Peroneal tendon displacement (subluxation or dislocation) accompanying an intra-articular calcaneal fracture is often undetected and under-treated. The goals of this study were to determine (1) the prevalence of peroneal tendon displacement accompanying intra-articular calcaneal fractures, (2) the association of tendon displacement with fracture classifications,

(3) the association of tendon displacement with heel width, and (4) the rate of missed diagnosis of the tendon displacement on radiographs and computed tomography (CT) scans and the resulting treatment rate.

Methods: A retrospective radiographic review

of all calcaneal fractures presenting at three institutions from June 30, 2006, to June 30, 2011, was performed. CT imaging of 421 intra-articular calcaneal fractures involving the posterior facet was available for review. The prevalence of peroneal tendon displacement was noted and its associations with fracture classification and heel width were evaluated.

Results: Peroneal tendon displacement was identified in 118(28.0%) of the 421 calcaneal fracture cases. The presence of tendon displacement was significantly associated with joint-depression fractures compared with tongue-type fractures (p < 0.001). Only twelve (10.2%) of the 118 AZD8055 cases of peroneal tendon displacement had been identified in the radiology reports. Although sixty-five (55.1%) of the fractures with tendon displacement had been treated with internal fixation, the tendon displacement was treated surgically in only seven (10.8%) of these cases.

Conclusions: Analysis of CT images showed

a 28% prevalence of peroneal tendon displacement accompanying intra-articular calcaneal fractures. Surgeons and radiologists Quisinostat mouse are encouraged to consider this association.”
“Dyskeratosis congenita (DC) is a rare inherited condition, first described in 1960 as Zinsser-Engmann-Cole syndrome. The condition presents with a classic triad: nail dystrophy, reticulate skin pigmentation over the trunk and neck, and white plaques typically in the oral cavity. White plaques may also affect, although less frequently, the mucosal surfaces of the urethra and vagina. Variable somatic abnormalities may be present; these include: pulmonary, gastrointestinal, genitourinary, cerebral, and dental complications. Pancytopenia eventually develops, progressing to bone marrow failure; the most common cause of death. In the X-linked form, which affects mainly males, mutations arise in the DKC-1 gene which encodes for the protein dyskerin. This report details a variant case of DC affecting a 9-year-old boy. The disease progression did not follow the “”classic triad”" typically seen in the condition.

This suggests that self-rated health reflects a broad range of factors important for development of this cancer type. Nevertheless, due to the explorative analysis of the specific cancer types, these findings need to be repeated before elaborate interpretations can be made.”
“Limited information is available on the use of botulinum toxin type A injections for children with hereditary spastic IACS-10759 chemical structure paraplegia. This report includes 12 children with hereditary spastic paraplegia (mean age 4.8 +/- 2.5 years) who underwent 1 to 6 sessions of botulinum toxin A injections to the hamstrings, adductors and gastrocnemius muscles. Patients showed both improved muscle tone (mean 1.9 +/- 0.5 vs 1.18 +/- 0.33,

P < .001, Ashworth Scale) and motor function (75.3 +/- 11.9 vs 77.7 +/- 11, P < .001, Gross Motor Function Measure). The effect lasted for a mean of 6.6 +/- 3.6 months. During the study period (mean 2.8 +/- 1.8 years), the preinjection

Gross Motor Function Measure increased (69.2 +/- 14.7 vs 78.3 +/- 13.5, P = .005), whereas the Ashworth Scale remained stable, suggesting a prolonged effect of botulinum toxin A on motor function. The authors conclude that botulinum toxin A injections to Talazoparib lower limbs of pediatric patients with hereditary spastic paraplegia result in prolonged functional improvement despite the progressive nature of the disease.”
“The aim of the study is to investigate whether the presence of a protein Z polymorphism is a risk factor for the development and outcome of sepsis. Sepsis is a clinical syndrome characterized by the presence of systemic signs and symptoms of inflammation. When sepsis leads to organ failure, the term severe sepsis and septic shock is used. The genetic causes of severe sepsis are not fully explained. Protein Z is a vitamin K-dependent glycoprotein and a member of the coagulation cascade. The study included 53 patients with severe sepsis and 70 control healthy volunteers without a familial history of thrombosis. The G79A polymorphism of intron F of the protein Z gene was analyzed by the method of polymerase chain reaction-based

DNA analysis. The protein Z intron F G79A polymorphism frequencies of the patients and controls were 43.4% and 40%, respectively. Carrying 79 AA genotype could www.sellecn.cn/products/Everolimus(RAD001).html be a risk factor for severe sepsis and septic shock (OR 4.5, 95% CI: 0.45-46.1), but it could not find any difference between survivor and nonsurvivor groups. They concluded that the frequency of intron F G79A polymorphism of protein Z gene was higher in patients than controls, and carrying 79 AA genotype could be a risk factor for severe sepsis and septic shock.”
“Background: Mothers may be reluctant to receive medical treatment of postpartum depression (PPD), despite the detrimental consequences the disorder can impose on mother and child. Research on alternative methods of prevention and treatment of PPD is warranted.

Results: The prevalence of US adults in the best possible EQ-5D state (i.e., 11111) was lower for the Y (38%) and 5L (35%) than for the 3L (44%), capturing more health problems. However, the prevalence of extreme responses in pain/discomfort and anxiety/depression decreased substantially between the 3L and 5L (from 44% to 17% and from 29% to 13%, respectively).

Conclusion: Compared

with the 3L, the Y and 5L versions SCH772984 order describe population health as having more, yet milder, health problems. Although the 5L may have advantages in patient populations in which extreme problems are more prevalent, population studies or studies that follow patients from childhood may consider using the Y. (C) 2014 Elsevier Inc. All Selleckchem ARS-1620 rights reserved.”
“Objective.

The present study aimed to assess neuropathic symptoms, their stability over time and relationship to pain intensity, pain distribution, and emotional distress in patients with musculoskeletal disorders.

Design.

This

is a prospective study.

Setting.

The study was done at the Department of Physical Medicine and Rehabilitation at Ulleval University Hospital.

Patients.

Eighty-six subjects between 18 years and 70 years with chronic musculoskeletal pain participated. Forty-nine subjects had widespread pain and 39 subjects fulfilled the American College of Rheumatology (ACR) criteria for fibromyalgia.

Outcome Measures.

McGill selleck chemical pain drawing, pain intensity (visual analog scales), emotional distress (Hopkins Symptom Checklist v 25), and fibromyalgia impact questionnaire were the recorded predictors, and neuropathic symptoms (Leeds assessment of neuropathic symptoms and signs-LANSS) were the main outcome variable which was assessed over 4 months.

Results.

The mean LANSS score was 6.7 (standard deviation 5.6). Thirteen percent of the subjects had a score of 12 or more. Self-reported LANSS symptoms did not change over the 4 months follow-up, and the reliability of measurements as evaluated

by intraclass correlation coefficient was 0.78. In a backward multiple regression analysis, the presence of fibromyalgia diagnosis and emotional distress remained the final predictors for neuropathic symptoms.

Conclusions.

Our study demonstrates that neuropathic symptoms are prominent features of chronic musculoskeletal pain and are stable over time. These symptoms were closely related to emotional distress and to the diagnosis of fibromyalgia. The results lend support to the theory that neuropathic symptoms represent an underlying sensitization.”
“Objectives: Quality of Life Questionnaire Core 30 (QLQ-C30) and Functional Assessment of Cancer Therapy General (FACT-G) are widely used cancer-specific health-related quality of life (HRQOL) questionnaires. We aimed to compare their responsiveness with clinically important effects and statistical efficiency to detect such effects.

Therefore, mitochondrial function has to be carefully balanced in accordance with the developmental stage and metabolic requirements of the cell. One way to adapt organellar function is the alteration of protein composition. Since most mitochondrial proteins are nuclear encoded, fine-tuning of mitochondrial protein content could be achieved by the regulation

of protein translocation. Here we present evidence that the import of nuclear-encoded mitochondrial Daporinad datasheet proteins into plant mitochondria is influenced by calcium and calmodulin. In pea mitochondria, the calmodulin inhibitor ophiobolin A as well as the calcium ionophores A23187 and ionomycin inhibit translocation of nuclear-encoded proteins in a concentration-dependent manner, an effect that can be countered by the addition of external calmodulin or calcium, respectively. Inhibition was observed exclusively for proteins translocating into or across the inner membrane but not for proteins residing in the outer membrane or the intermembrane space. Ophiobolin A and the calcium

ionophores further inhibit translocation into mitochondria with disrupted outer membranes, but their effect is not mediated via a change in the membrane potential across the inner mitochondrial membrane. Together, our results suggest that calcium/calmodulin influences the import of a subset of mitochondrial proteins at the inner membrane. Interestingly, we could not observe any influence of ophiobolin A or the calcium GS-4997 cell line ionophores on protein translocation into mitochondria of yeast, indicating that the effect buy Volasertib of calcium/calmodulin on mitochondrial protein import might be a plant-specific

trait.”
“BACKGROUNDThe models presented for bed optimum fluidization velocity (u(opt)), which was worthwhile for treatment efficiency and operation stability of anaerobic fluidized-bed bioreactors (AFB), were developed for AFB, and the anaerobic granular sludge diameter (d(p)) and shape were analyzed by a dynamic image analyzer.

RESULTSThe results showed that the granular size from 0.05 to 0.30 cm sludge dominated the total biomass (accounted for 92.34%) in an expanded granular sludge bed reactor, the mean sphericity 0.835 and mean granular diameter 0.1606 cm were achieved. The u(opt) range was 0.000949-0.2851 cm s(-1) when d(p) was 0.05-0.30 cm, and the fluidization number was 8.43-38.77. The maximum value of 0.2851 cm s(-1) was the basic parameter for pump power matching. From parametric sensitivity analyses, u(opt) was significantly influenced by liquid holdup, granular diameter, granular density and liquid viscosity.

CONCLUSIONAccording to the models of u(opt), a better operating scheme for AFB can be provided; from parametric sensitivity analysis, further optimizing designs of such bioreactors can be achieved.

Renal variables were measured

at baseline and 15 and 60 min after resuscitation. Results. Hemorrhage induced reductions of mean arterial pressure, filling pressures, and cardiac index (p 0.05), without significant differences among groups (p 0.05). After 60 min of shed blood replacement, all groups restored hemodynamic and renal variables PX-478 purchase to the prehemorrhage levels (p 0.05), without significant differences among groups (p 0.05), with the exception of sodium fractional excretion, the values for which were significantly higher in isoflurane group, in relation to the other groups after 15 min of re-transfusion (p 0.05), and renal vascular resistance, the values for which remain lower than baseline in halothane group (p 0.05). Conclusions. We conclude that no difference could

be detected between choosing equipotent doses of halothane, sevoflurane, or isoflurane in relation to renal variables in dogs submitted to pressure-adjusted hemorrhagic shock and resuscitation.”
“Our aims were to correlate transforming growth factor (TGF)-beta 1 and elastin mRNA expression in the vagina of women and to measure the effects of TGF-beta 1 on vaginal smooth muscle cell (SMC) proliferation and tropoelastin buy Selisistat production.

Vaginal walls were sampled in women (n = 20). TGF-beta 1 and elastin mRNA expression was assessed by RT-PCR. SMC cultures were performed from vaginal wall biopsies. SMC were incubated with TGF-beta 1, and cell proliferation Nocodazole Cytoskeletal Signaling inhibitor was assessed by MTT-assay. Tropoelastin production was measured by the Fastin Elastin Assay.

There was a significant positive correlation between TGF-beta 1 and elastin mRNA (r = 0.784, P < 0.01). SMC proliferation was significantly increased by 10 ng/mL TGF-beta 1 [relative cell number, mean +/- SD, 198% +/- 32% of control (P = 0.01)]. Tropoelastin production was significantly increased by TGF-beta 1 [mean +/- SD, 645% +/- 180% of control (P = 0.01)].

There is a positive correlation between TGF-beta 1 and elastin mRNA expression in the vaginal wall. In vitro, TGF-beta 1

increases vaginal tropoelastin production in vaginal SMC.”
“Background: The consumption of dairy products may influence the risk of cardiovascular disease (CVD) and total mortality, but conflicting findings have been reported.

Objective: The objective was to examine the associations of milk, total dairy products, and high- and low-fat dairy intakes with the risk of CVD [including coronary heart disease (CHD) and stroke] and total mortality.

Design: PubMed, EMBASE, and SCOPUS were searched for articles published up to February 2010. Of >5000 titles evaluated, 17 met the inclusion criteria, all of which were original prospective cohort studies. Random-effects meta-analyses were performed with summarized dose-response data. Milk as the main dairy product was pooled in these analyses.

In recent years, considerable progress has been made in our understanding of many mendelian diseases caused by mutations in one or a small number of genes. However, it is proving much more difficult to elucidate the genetic basis of complex diseases, including BTSA1 nmr coronary heart disease (CHD). The objective of this paper was to review some of the most significant findings observed to date in studies into the genetic component of CHD. We report on the most important results from linkage and association studies, including genome-wide association studies. Finally, we summarize the potential usefulness of these findings for daily clinical practice and disease prevention, and look

forward to the challenges we can expect to encounter in the near future.”
“Objective-To

characterize a 2007 bluetongue disease (BT) epizootic caused by bluetongue virus (BTV) serotype 17 in sheep in the Big Horn Basin of Wyoming.

Design-Cross-sectional study.

Animals-1,359 sheep from ranches in Wyoming and Montana.

Procedures-Information on clinical signs and history of BT in sheep was obtained from ranchers and attending veterinarians. At 3 to 6 months after the 2007 BT epizootic, blood samples were collected from rams, ewes, and lambs within and outside the Big Horn Basin; blood samples were also collected from lambs born in the spring of 2008. Sera were tested for anti-BTV antibodies by use of a competitive ELISA to determine the seroprevalence of BTV in sheep and to measure antibody titers. Virus isolation Daporinad supplier and reverse transcriptase PCR assays were used to determine long-term presence of the infectious virus or viral genetic material in RBCs of sheep.

Results-The percentage

of sheep seropositive for BTV closely matched morbidity of sheep within flocks, indicating few subclinical infections. Flocks separated by as little as 1 mile had substantial variation AZD1080 research buy in infection rate. Rams were infected at a higher rate than ewes. There was no evidence of BTV successfully overwintering in the area.

Conclusions and Clinical Relevance-This epizootic appears to be a new intrusion of BTV into a naive population of sheep previously protected geographically by the mountains surrounding the Big Horn Basin. Rams may have a higher infection rate as a result of increased vector biting opportunity because of the large surface area of the scrotum. Am Vet Med Assoc 2010;237:955-959)”
“The landscape of the treatment of relapsing-remitting multiple sclerosis is changing fast. Several oral treatments have shown benefit and generate much interest because of the convenience of their administration. Two oral compounds, fingolimod and teriflunomide, have been approved in relapsing-remitting multiple sclerosis, while others have completed Phase III trials and are awaiting review for registration.

However, this involvement may differ among the animal strains. We thus examined GPCR Compound Library the impact of the genetic background on the role of MBL in diabetic nephropathy. Materials/Methods. C57BL/6JBomTac and 129S6/SvEvTac mice were compared. In both strains, experimental type 1 diabetes was induced in wild-type (WT) and MBL-knockout (MBL-KO) mice by streptozotocin. Nondiabetic WT and MBL-KO mice were used

as controls. We tested if MBL modified the diabetes-induced kidney changes by two-way ANOVA allowing for interaction. Results. MBL aggravated diabetes-induced kidney growth and glomerulus enlargement in C57BL/6JBomTac mice. MBL did not modify diabetes effects on glomerular basement membrane thickness or mesangial volume

in any strain. Diabetes-induced changes in renal gene transcription of growth factors and matrix components were unaffected by MBL. Conclusions. Strain-specific MBL effects were found on downstream diabetic kidney changes. This emphasizes the importance of genetic background in this model of diabetic complications.”
“Background: The Plasmodium SRT2104 supplier purine salvage enzyme, hypoxanthine guanine xanthine phosphoribosyl transferase (HGXPRT) can protect mice against Plasmodium yoelii pRBC challenge in a T cell-dependent manner and has, therefore, been proposed as a novel vaccine candidate. It is not known whether natural exposure to Plasmodium falciparum stimulates HGXPRT T cell reactivity in humans.

Methods: PBMC and plasma collected from malaria-exposed Indonesians during infection and 7-28 days after anti-malarial therapy, were assessed for HGXPRT recognition using CFSE proliferation, IFN gamma ELISPOT assay and ELISA.

Results: HGXPRT-specific T cell proliferation was found in 44% of patients during acute infection; in 80% of responders both CD4(+) and CD8(+) T cell subsets proliferated. Antigen-specific T cell proliferation was largely lost within 28 days of parasite clearance. HGXPRT-specific IFN-gamma production was more frequent 28 days after treatment than during acute infection.

HGXPRT-specific plasma IgG MK-8776 chemical structure was undetectable even in individuals exposed to malaria for at least two years.

Conclusion: The prevalence of acute proliferative and convalescent IFN gamma responses to HGXPRT demonstrates cellular immunogenicity in humans. Further studies to determine minimal HGXPRT epitopes, the specificity of responses for Plasmodia and associations with protection are required. Frequent and robust T cell proliferation, high sequence conservation among Plasmodium species and absent IgG responses distinguish HGXPRT from other malaria antigens.”
“Nanoscale-phase separation of electron donor/acceptor blends is crucial for efficient charge generation and collection in Polymer bulk heterojunction photovoltaic cells.

Overall survival was better for patients who had stage II disease before implant placement than for those who had stage III disease and also for patients who had relatively less parametrial disease. Grade 4 complications were seen only in four patients and were within acceptable limits. Multivariate

analysis showed that only the BPF https://www.selleckchem.com/products/xmu-mp-1.html variable had a statistically significant effect on survival. Conclusion: We conclude that reirradiation with HDR brachytherapy implant in recurrent or residual carcinoma cervix can give a good chance of survival, with acceptable morbidity, if proper selection of patients is done. This treatment should not be done for palliative purposes.”
“An analytic model addressing the energy bandgap of nanoporous (NPs) structures with aligned cylindrical pores has been established from the perspective of nanothermodynamic considerations. It is found that the bandgap energies of NPs structures are unambiguously blueshifted as compared

with those of nanowires with the same sizes and the bulk counterparts. high throughput screening compounds The anomalous surface energies in the inner and outer surfaces of NPs structures seem the physical origin of the bandgap shifts. Agreement between the theoretical predictions and the evidence from experimental measurements and calculations suggested that the proposed method could be expected to be applicable to NPs structural materials. (C) 2011 American Institute of Physics. [doi:10.1063/1.3615974]“
“Modeling the different phases of the bacterial growth curve and the production of alpha-amylase by Bacillus Vorasidenib sp. KR-8104 in a solid-state fermentation process based on variation in dry weight was the aim of this study. The result showed that the growth of bacterial cells

and the production of alpha-amylase on a wheat bran substrate could be expressed by simple models incorporating the mathematical definition of each phase and the variation in dry substrate weight over the incubation time. Experimental data collected from a series of batch fermentations were used to validate the models proposed. In both cases, the model simulation matched well with the experimental observations, which made it possible to conclude that these models can be successfully employed for the development of growth and alpha-amylase production in solid-state fermentation processes. (C) 2010 Elsevier B.V. All rights reserved.”
“Background: An appropriate animal model for malignant melanoma could be a strong tool to develop biomarkers through analysis of melanomagenesis.

Objective: Development of a novel animal model that spontaneously develops malignant melanoma with a high percentage.